3FR8
rice Ketolacid reductoisomerase in complex with Mg2+-NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 162.060, 162.060, 196.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.220 - 2.800 |
| R-factor | 0.23564 |
| Rwork | 0.233 |
| R-free | 0.28398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yve |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.046 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.220 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 | 0.020 |
| Number of reflections | 31711 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.0 | 99.8 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M TRIS-HCl pH 8.5, 1M (NH4)2SO4, 15% (w/v) polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
