3FQJ

Crystal Structure of the Mouse Dom3Z in Complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-08
Detector	Mar CCD M-165
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	58.293, 73.573, 108.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.150 - 2.623
R-factor	0.207
Rwork	0.204
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.131	0.077	0.489
Number of reflections	14312
<I/σ(I)>	11.052
Completeness [%]	98.7	96.8	97.7
Redundancy	3.4	3.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.2 M potassium phosphate monobasic and 25% (w/v) PEG 3350, pH 7.5, vapor diffusion, sitting drop, temperature 295K