3FQG
Crystal Structure of the S. pombe Rai1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-18 |
| Detector | Mar m-165 CCD |
| Wavelength(s) | 0.981 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.597, 60.790, 73.124 |
| Unit cell angles | 90.00, 103.01, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.079 | 0.049 | 0.376 |
| Number of reflections | 29188 | ||
| <I/σ(I)> | 16.785 | ||
| Completeness [%] | 97.9 | 97.8 | 91.1 |
| Redundancy | 3.1 | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 0.2 M sodium citrate tribasic (pH 5.0) and 20% (w/v) PEG 3350, vapor diffusion, sitting drop, temperature 295K |
