3FQ6
The crystal structure of a methyltransferase domain from Bacteroides thetaiotaomicron VPI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926, 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.321, 70.919, 86.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.500 - 2.360 |
| R-factor | 0.21694 |
| Rwork | 0.214 |
| R-free | 0.28571 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.716 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.088 | 0.528 |
| Number of reflections | 11310 | |
| <I/σ(I)> | 30.7 | 2.68 |
| Completeness [%] | 95.8 | 80.9 |
| Redundancy | 5.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M Ammonium Sulfate, 0.1M Bis-Tris, 25% w/v PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
