3FQ4
Crystal structure of the Calx-beta domain of integrin beta4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.310, 51.660, 87.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.830 - 1.487 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on PDB entry 2DPK |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.810 | 1.540 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 38089 | |
| <I/σ(I)> | 29.5 | 8.6 |
| Completeness [%] | 98.3 | 85.4 |
| Redundancy | 13.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 50mM Tris-HCl, 26% PEG 1500, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
