3FPU
The crystallographic structure of the Complex between Evasin-1 and CCL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 104.384, 104.384, 104.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.760 |
| R-factor | 0.23398 |
| Rwork | 0.231 |
| R-free | 0.28467 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fpr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 19881 | |
| <I/σ(I)> | 13.7 | 7.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 40.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 291 | 24% (w/v) PEG 3350, 200mM Ammonium sulfate, 100mM HEPES, pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
