3FPT
The Crystal Structure of the Complex between Evasin-1 and CCL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.704, 70.491, 103.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 2.700 |
| R-factor | 0.23005 |
| Rwork | 0.226 |
| R-free | 0.30521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fpr |
| RMSD bond length | 0.045 |
| RMSD bond angle | 4.049 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 26472 | |
| <I/σ(I)> | 0.152 | 3.6 |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 23% (w/v) PEG 4000, 300mM Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
