3FPK
Crystal Structure of Ferredoxin-NADP Reductase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.423, 69.989, 109.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 1.700 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fdr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.688 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.768 |
| Number of reflections | 59191 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 99.1 | 90.2 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Calcium chloride, 0.1 M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
