3FP0
Structural and Functional Characterization of TRI3 Trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-06 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.282, 81.987, 95.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.140 - 1.900 |
| R-factor | 0.18319 |
| Rwork | 0.180 |
| R-free | 0.23538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fot |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.654 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 40098 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 98.0 | 90 |
| Redundancy | 4.96 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 2M Ammonium sulfate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
