3FOU
Low pH structure of the Rieske protein from Thermus thermophilus at 2.1 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.152, 58.512, 58.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.800 - 2.100 |
| R-factor | 0.20072 |
| Rwork | 0.198 |
| R-free | 0.24601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Protein A of 1NYK |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.800 | 2.154 |
| High resolution limit [Å] | 2.100 | 2.099 |
| Number of reflections | 23456 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.21 | 298 | PEG 8000, Na Cacodylate, Calcium acetate, Pr(acetate)3, pH 6.21, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
