3FOQ
Crystal structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-24 |
Detector | MAR345 |
Wavelength(s) | 1.54179 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 285.700, 285.700, 285.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.970 - 3.410 |
R-factor | 0.2872 |
Rwork | 0.285 |
R-free | 0.32149 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 1.936 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.590 |
High resolution limit [Å] | 3.410 | 3.500 |
Rmerge | 0.194 | 0.552 |
Number of reflections | 26978 | 1785 |
<I/σ(I)> | 4.4 | |
Completeness [%] | 99.0 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.8M Ammonium sulphate, 25mM MES, 5% Glycerol, 5mM MgCL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |