3FOJ

Crystal Structure of SSP1007 From Staphylococcus saprophyticus subsp. saprophyticus. Northeast Structural Genomics Target SyR101A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	30.692, 42.130, 58.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.210 - 1.600
R-factor	0.194
Rwork	0.194
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.076
Number of reflections	19428
<I/σ(I)>	17.2	16
Completeness [%]	99.7	99.4
Redundancy	6.1	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	150mM MgSO4, 100 MM Na3 Citrate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K