3FNN
Biochemical and structural analysis of an atypical ThyX: Corynebacterium glutamicum NCHU 87078 depends on ThyA for thymidine biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 95.806, 95.806, 64.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.430 - 2.300 |
| R-factor | 0.22453 |
| Rwork | 0.223 |
| R-free | 0.25952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2af6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.045 | 0.492 |
| Number of reflections | 13929 | |
| <I/σ(I)> | 3.61 | |
| Redundancy | 9.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
