3FNL
Crystal Structure of the Complex of Buffalo Lactoperoxidase with Salicylhydroxamic Acid at 2.48 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.479, 80.632, 78.121 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.480 |
| R-factor | 0.20239 |
| Rwork | 0.200 |
| R-free | 0.24001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z5z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.442 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 22068 | |
| <I/σ(I)> | 5.8 | 1.2 |
| Completeness [%] | 92.3 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 290 | TRIS HCL, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
