3FN9
Crystal structure of putative beta-galactosidase from bacteroides fragilis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.453, 133.066, 221.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.363 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.146 | 0.065 | 0.833 |
| Number of reflections | 89259 | ||
| <I/σ(I)> | 16.844 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 10.3 | 10.2 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-tris pH 6.5, 25% PEG 3350, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
