3FMW
The crystal structure of MtmOIV, a Baeyer-Villiger monooxygenase from the mithramycin biosynthetic pathway in Streptomyces argillaceus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-04-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.265, 114.437, 138.563 |
| Unit cell angles | 90.00, 103.03, 90.00 |
Refinement procedure
| Resolution | 29.680 - 2.890 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qa1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.993 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.563 |
| High resolution limit [Å] | 2.887 |
| Number of reflections | 48046 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 0.1 M Na-HEPES pH 7.5, 10%(v/w) PEG 6000 and 5%(v/v) MPD, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
