3FMV
Crystal structure of the serine phosphatase of RNA polymerase II CTD (SSU72 superfamily) from Drosophila melanogaster. Monoclinic crystal form. Northeast Structural Genomics Consortium target FR253.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97920, 0.97947, 0.96791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.983, 98.995, 158.123 |
| Unit cell angles | 90.00, 90.87, 90.00 |
Refinement procedure
| Resolution | 19.930 - 3.310 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.121 | 0.477 |
| Number of reflections | 54833 | |
| <I/σ(I)> | 14 | 2.7 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 6.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 18% PEG 3350, 2% Benzamidine, 0.2M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
