3FM8
Crystal structure of full length centaurin alpha-1 bound with the FHA domain of KIF13B (CAPRI target)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.632, 115.632, 189.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.865 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished model of human centaurin alpha-1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.259 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.930 | 2.300 |
| Rmerge | 0.080 | 0.038 | 0.739 |
| Number of reflections | 57550 | ||
| Completeness [%] | 99.7 | 98.8 | 98.3 |
| Redundancy | 7.1 | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 1.2M lithium sulfate, 0.1M Sodium citrate, 0.5M ammonium sulfate, pH 6.0, vapor diffusion, hanging drop, temperature 291K |
