3FM6
Crystal Structure Analysis of Fungal Versatile Peroxidase from Pleurotus eryngii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97992 |
| Spacegroup name | P 43 |
| Unit cell lengths | 63.333, 63.333, 99.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.323 - 1.130 |
| R-factor | 0.1004 |
| Rwork | 0.100 |
| R-free | 0.11482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fkg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.160 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.085 | 0.130 |
| Number of reflections | 145501 | |
| <I/σ(I)> | 13.5 | 7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.1 | 3.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 9.0mg/ml protein in 10mM Na-tartrate pH 5.5, 16% PEG 8000, 100mM Zn-acetate, 100mM Na-cacodylate pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
