3FM4
Crystal Structure Analysis of Fungal Versatile Peroxidase from Pleurotus eryngii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8065 |
| Spacegroup name | P 43 |
| Unit cell lengths | 62.794, 62.794, 97.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.880 - 2.110 |
| R-factor | 0.14418 |
| Rwork | 0.141 |
| R-free | 0.19776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fkg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.379 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.150 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Number of reflections | 21485 | |
| <I/σ(I)> | 9.1 | 1.7 |
| Completeness [%] | 97.6 | 78.5 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 9.0mg/ml protein in 10mM Na-tartrate pH 5.5, 16% PEG 8000, 100mM Zn-acetate, 100mM Na-cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
