3FM1
Crystal Structure Analysis of Fungal Versatile Peroxidase from Pleurotus eryngii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 43 |
| Unit cell lengths | 63.645, 63.645, 99.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.710 - 1.780 |
| R-factor | 0.12675 |
| Rwork | 0.124 |
| R-free | 0.17241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fkg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.622 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 40092 | |
| <I/σ(I)> | 13.2 | 2.8 |
| Completeness [%] | 98.6 | 99.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 9.0mg/ml protein in 10mM Na-tartrate pH 5.5, 17% PEG 10000, 200mM Zn-acetate, 100mM Na-cacodylate pH 6.5; Crystals were soaked with Mn2+; VAPOR DIFFUSION, HANGING DROP, temperature 298K |
