3FM0
Crystal structure of WD40 protein Ciao1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.682, 74.640, 49.481 |
| Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.700 |
| R-factor | 0.16139 |
| Rwork | 0.160 |
| R-free | 0.19589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hes |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.421 |
| Number of reflections | 30710 | |
| <I/σ(I)> | 12.5 | 2.62 |
| Completeness [%] | 95.7 | 66.5 |
| Redundancy | 5.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 297 | 25% PEG 3350, 0.1M (NH4)2SO4, 0.1M Bis-tris, pH6.0, 8% 2,2,2-trifluro ethanol, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
