3FLV
The crystal structure of human acyl-CoenzymeA binding domain containing 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99988 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.751, 34.064, 54.877 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 28.780 - 1.700 |
| R-factor | 0.21458 |
| Rwork | 0.212 |
| R-free | 0.25926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbk |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.571 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.780 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.539 |
| Number of reflections | 25392 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 91.8 | 54.3 |
| Redundancy | 6.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M Mg(ac)2; 0.1M cacodylate pH 6.5; 20% PEG 8K, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
