3FLJ

Crystal structure of uncharacterized protein conserved in bacteria with a cystatin-like fold (YP_168589.1) from SILICIBACTER POMEROYI DSS-3 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97982
Spacegroup name	I 2 3
Unit cell lengths	120.880, 120.880, 120.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.490 - 2.000
R-factor	0.177
Rwork	0.176
R-free	0.18500
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.711
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.490	28.490	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.072	0.020	0.866
Number of reflections	19903	3845	3591
<I/σ(I)>	11.95	41.2	1.4
Completeness [%]	99.1	98.7	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2000M MgCl2, 2.5000M NaCl, 0.1M TRIS pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K