3FLE
SE_1780 protein of unknown function from Staphylococcus epidermidis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.722, 41.458, 153.996 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 26.995 - 2.009 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.050 |
| High resolution limit [Å] | 2.009 | 2.020 |
| Rmerge | 0.114 | 0.524 |
| Number of reflections | 77366 | |
| <I/σ(I)> | 15.682 | 2 |
| Completeness [%] | 98.2 | 92.1 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 20% PEG 1000, 0.1 M Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
