3FKW
AmpC K67R mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.348, 76.564, 97.702 |
| Unit cell angles | 90.00, 115.82, 90.00 |
Refinement procedure
| Resolution | 88.050 - 1.498 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1KE4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 88.050 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.498 | 3.230 | 1.498 |
| Rmerge | 0.051 | 0.035 | 0.513 |
| Number of reflections | 120185 | ||
| <I/σ(I)> | 16.662 | ||
| Completeness [%] | 95.7 | 98.4 | 90.9 |
| Redundancy | 2.5 | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | Potassium Phosphate, pH 8.7, vapor diffusion, hanging drop, temperature 293K |
