3FKE

Structure of the Ebola VP35 Interferon Inhibitory Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-30
Detector	NOIR-1
Wavelength(s)	0.9792, 0.9795, 0.9643
Spacegroup name	P 21 21 21
Unit cell lengths	51.490, 66.210, 72.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.400
R-factor	0.20159
Rwork	0.198
R-free	0.23647
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.584
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.910	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.061	0.441
Number of reflections	47946
<I/σ(I)>	11	2.4
Completeness [%]	99.7	98.8
Redundancy	6.65	4.91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	Well solution: 200 mM Sodium Citrate (pH 5.8),11% (W/V) PEG 4000; protein solution: 20 mM Tris-Cl (pH 7.0),50 mM NaCl, 5mM beta-mercaptoethanol, Vapor Diffusion, Hanging Drop, Temperature 298K, VAPOR DIFFUSION, HANGING DROP