3FKD
The crystal structure of L-threonine-O-3-phosphate decarboxylase from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.443, 101.513, 93.714 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 46.857 - 2.500 |
| R-factor | 0.2266 |
| Rwork | 0.224 |
| R-free | 0.27450 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 0.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | 0.313 |
| Number of reflections | 56150 | |
| <I/σ(I)> | 12.4 | 3.9 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 0.1 M Sodium citrate tribacic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP |
