3FKA
CRYSTAL STRUCTURE OF A NTF-2 LIKE PROTEIN OF UNKNOWN FUNCTION (SPO1084) FROM SILICIBACTER POMEROYI DSS-3 AT 1.69 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94645,0.97967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.424, 92.002, 120.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.961 - 1.690 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.917 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.961 | 29.960 | 1.730 |
| High resolution limit [Å] | 1.690 | 7.560 | 1.690 |
| Rmerge | 0.080 | 0.047 | 0.629 |
| Rmeas | 0.093 | 0.056 | 0.744 |
| Total number of observations | 231193 | 2561 | 11241 |
| Number of reflections | 62487 | ||
| <I/σ(I)> | 11 | 25.6 | 1.9 |
| Completeness [%] | 95.2 | 97.9 | 71.2 |
| Redundancy | 3.7 | 3.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2000M Ca(OAc)2, 10.0000% PEG-8000, 0.1M Imidazole pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
