3FK5

Crystal structure of 3-oxoacyl-(acyl carrier protein) synthase III, FabH (Xoo4209) from Xanthomonas oryzae pv. oryzae KACC10331

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	69.750, 79.450, 62.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 2.050
R-factor	0.17722
Rwork	0.174
R-free	0.23692
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hnj
RMSD bond length	0.022
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	20632
Completeness [%]	100.0	81.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	287	0.1M HEPES pH 7.2, 30% PEG 6000, 5% 2-methyl-2, 4-pentanediol, 3% D-galactose , VAPOR DIFFUSION, SITTING DROP, temperature 287K