3FJW
Crystal Structure Analysis of Fungal Versatile Peroxidase from Pleurotus eryngii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.8499 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.141, 92.762, 113.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.800 |
| R-factor | 0.16277 |
| Rwork | 0.158 |
| R-free | 0.24629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qpa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.747 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.350 |
| Number of reflections | 19316 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8.0mg/ml protein in 10mM Na-citrate pH 4.0, 1.9M ammonium sulphate, 6% PEG 400, 100mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
