3FJP
Apo structure of Biotin protein ligase from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.533, 61.342, 73.283 |
| Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
| Resolution | 30.867 - 2.300 |
| R-factor | 0.2229 |
| Rwork | 0.220 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wpy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.368 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20759 | |
| <I/σ(I)> | 22.4 | 5 |
| Completeness [%] | 92.4 | 64.6 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, 0.2M AMMONIUM SULPHATE, 15% MPEG 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
