3FIX

Crystal structure of a putative n-acetyltransferase (ta0374) from thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	42.229, 60.915, 72.116
Unit cell angles	101.19, 90.10, 89.97

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.23215
Rwork	0.230
R-free	0.27555
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.999
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.5.0051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.380
Number of reflections	30257
Completeness [%]	97.1	91.2
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M HEPES, 10% (w/v) PEG 8000, 10% (w/v) ETHYLENE GLYCOL , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K