3FIV
CRYSTAL STRUCTURE OF FELINE IMMUNODEFICIENCY VIRUS PROTEASE COMPLEXED WITH A SUBSTRATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-23 |
| Detector | MACSCIENCE |
| Spacegroup name | P 31 |
| Unit cell lengths | 50.890, 50.890, 74.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fiv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 27.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 * | |
| Total number of observations | 40465 * | |
| Number of reflections | 17411 * | |
| <I/σ(I)> | 16 | 2.59 |
| Completeness [%] | 87.2 | 68.5 * |
| Redundancy | 2.32 | 1.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 2.0 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, PH=5.6. PROTEIN CONCENTRATION, 5.0 MG/ML. CRYSTALLIZATION METHOD: HANGING DROP VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | FIV PR | 0.0025ml | |
| 2 | 1 | drop | LP-149 | 7 (mg/ml) | |
| 3 | 1 | drop | substrate | 5 (mg/ml) | |
| 4 | 1 | drop | imidazol-hydrochloride | 50 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 8 | 1 | reservoir | sodium acetate | 0.1 (M) |
