3FIS

THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	79.400, 50.900, 47.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.300
R-factor	0.183
Rwork	0.183
RMSD bond length	0.017 *
RMSD bond angle	3.600 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.250 *
Rmerge	0.032 *
Total number of observations	30267 *
Number of reflections	6451 *
Completeness [%]	68.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop		1 (M)
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	2-mercaptoethanol	50 (mM)
5	1	reservoir	ammonium phosphate	1.3 (M)	can be replaced with PEG400