3FIM

Crystal structure of aryl-alcohol-oxidase from Pleurotus eryingii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	110
Detector technology	CCD
Collection date	2003-10-23
Detector	MARRESEARCH
Wavelength(s)	0.85
Spacegroup name	P 64 2 2
Unit cell lengths	180.226, 180.226, 160.407
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.040 - 2.550
R-factor	0.17507
Rwork	0.173
R-free	0.20640
Structure solution method	SAD
RMSD bond length	0.035
RMSD bond angle	2.693
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.700	37.700
High resolution limit [Å]	2.390	2.390
Rmerge	0.102	0.464
Number of reflections	56141
<I/σ(I)>		4
Completeness [%]	97.7	88.8
Redundancy	15	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	1M lithium sulfate, 0.1M Bis-Tris propane, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	1M lithium sulfate, 0.1M Bis-Tris propane, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	1M lithium sulfate, 0.1M Bis-Tris propane, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K