3FIA
Crystal structure of the EH 1 domain from human intersectin-1 protein. Northeast Structural Genomics Consortium target HR3646e.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.848, 32.021, 33.287 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 45.500 - 1.450 |
| R-factor | 0.16047 |
| Rwork | 0.160 |
| R-free | 0.17643 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.831 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.222 |
| Number of reflections | 31879 | |
| <I/σ(I)> | 33.75 | 4.9 |
| Completeness [%] | 96.1 | 99.3 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8.5 | 277 | 1.5M ammonium sulfate, 12% glycerol, 0.1M TrisHCl, pH 8.5, microbatch under oil , temperature 277K |
