3FI5

Crystal Structure of T4 Lysozyme Mutant R96W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-29
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	73.800, 56.110, 85.240
Unit cell angles	90.00, 106.47, 90.00

Refinement procedure

Resolution	18.804 - 1.530
R-factor	0.1955
Rwork	0.194
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lw9
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.804	1.570
High resolution limit [Å]	1.530	1.530
Rmerge	0.038	0.195
Number of reflections	92759
<I/σ(I)>	19.96	5.15
Completeness [%]	70.2	41.4
Redundancy	3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1