3FHV

Structural basis of Salmonella typhi type IVb PilS and cystic fibrosis transmembrane conductance regulator (CFTR) interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	77.799, 114.368, 31.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
Rwork	0.207
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fhu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	27226
<I/σ(I)>	26.4	6.12
Completeness [%]	98.5	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	4M Sodium Formate, VAPOR DIFFUSION, SITTING DROP, temperature 294'K