3FHP
A neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | NUCLEAR REACTOR |
| Source details | JRR-3M BEAMLINE 1G-B |
| Synchrotron site | JRR-3M |
| Beamline | 1G-B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-08 |
| Detector | MACSCIENCE |
| Wavelength(s) | 2.6 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.849, 82.849, 34.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.430 - 2.000 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.147 | 0.393 |
| Number of reflections | 4824 | |
| Completeness [%] | 23.2 | 81.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.3 | 293 | 2.3mg/ml insulin, 50mM sodium citrate, 6mM zinc acetate, 17% acetone, pH6.3, LIQUID DIFFUSION, temperature 293K |
