3FHO
Structure of S. pombe Dbp5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2008-05-07 |
| Wavelength(s) | 0.9725 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.736, 144.044, 79.137 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.29156 |
| Rwork | 0.290 |
| R-free | 0.32703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j0s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 | 0.343 |
| Number of reflections | 29528 | |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 98.5 | |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 288 | 100mM Tris, 200mM Ca(Ac)2, 8.5-10% PEG4000, pH 8.0, EVAPORATION, temperature 288K |
