3FHN
Structure of Tip20p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9794, 0.9640 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.480, 111.620, 149.840 |
| Unit cell angles | 77.09, 88.12, 70.35 |
Refinement procedure
| Resolution | 29.900 - 3.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 99867 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 98.5 | 98.2 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3:1 ratio of protein (2 mg ml-1) and well buffer (0.1 M ADA, pH 6.0, 10% (w/v) PEG monomethyl ether 5K, 0.2 M LiSO4, 3% (v/v) isopropanol, 5 mM DTT), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
