3FHF
Crystal structure of Methanocaldococcus jannaschii 8-oxoguanine DNA glycosylase (MjOgg)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794, 0.9796, 0.97166 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.440, 37.840, 75.010 |
| Unit cell angles | 90.00, 116.08, 90.00 |
Refinement procedure
| Resolution | 19.560 - 1.995 |
| R-factor | 0.222 |
| Rwork | 0.202 |
| R-free | 0.25100 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.560 | 2.100 |
| High resolution limit [Å] | 1.995 | 1.995 |
| Rmerge | 0.068 | 0.203 |
| Number of reflections | 28691 | |
| <I/σ(I)> | 15.43 | 8.23 |
| Completeness [%] | 96.4 | 92.1 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 285 | PEG 4000 30%, 0.1M sodium citrate, 1% b-Mercaptoethanol, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
