3FHD

Crystal structure of the Shutoff and Exonuclease Protein from Kaposis Sarcoma Associated Herpesvirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-07
Detector	MAR CCD 165 mm
Wavelength(s)	0.97954
Spacegroup name	P 1
Unit cell lengths	43.247, 48.905, 67.294
Unit cell angles	95.48, 106.86, 104.27

Refinement procedure

Resolution	46.630 - 1.850
R-factor	0.18582
Rwork	0.184
R-free	0.22807
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.350
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.630
High resolution limit [Å]	1.850
Rmerge	0.073
Number of reflections	41764
<I/σ(I)>	12.1
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M Lithium sulfate, 0.1M Bis-TRIS, 25%PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M Lithium sulfate, 0.1M Bis-TRIS, 25%PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M Lithium sulfate, 0.1M Bis-TRIS, 25%PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K