3FH9
Crystal structure determination of indian flying fox (Pteropus giganteus) at 1.62 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-17 |
| Detector | MAR 345 dtp |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.969, 64.669, 64.766 |
| Unit cell angles | 90.00, 129.61, 90.00 |
Refinement procedure
| Resolution | 17.450 - 1.620 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.26306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.164 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 35677 | |
| <I/σ(I)> | 12.2 | |
| Redundancy | 2.58 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.002ml of protein solution with 0.002ml of well solution containing 40% of PEG 3350 as precipitant, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
