3FH3
Crystal structure of a putative ECF-type sigma factor negative effector from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.808, 79.628, 80.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.814 - 2.102 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25890 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.107 | 0.500 |
| Number of reflections | 18762 | |
| <I/σ(I)> | 15.1 | 1.9 |
| Completeness [%] | 92.5 | 73.6 |
| Redundancy | 4.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.01M Nickle Chloride, Tris pH 8.5, 18% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
