3FH1

Crystal structure of a ntf2-like protein of unknown function (mll8193) from mesorhizobium loti at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94645,0.97965
Spacegroup name	P 43 21 2
Unit cell lengths	45.110, 45.110, 136.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.904 - 1.600
R-factor	0.169
Rwork	0.167
R-free	0.20000
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.596
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.904	28.904	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.063	0.023	1.036
Number of reflections	19510	3605	3655
<I/σ(I)>	13.65	43.5	1.45
Completeness [%]	99.6	99.6	98
Redundancy	6.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	0.2000M (NH4)2HCitrate, 20.0000% PEG-3350, No Buffer pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K