3FH0

Crystal structure of putative universal stress protein KPN_01444 - ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-05
Detector	CUSTOM-MADE
Wavelength(s)	0.97921
Spacegroup name	P 31 2 1
Unit cell lengths	67.604, 67.604, 162.986
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.150
R-factor	0.1929
Rwork	0.191
R-free	0.22695
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.536
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.5.0054)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.150	2.150
Rmerge	0.075	0.402
Number of reflections	24323
<I/σ(I)>	35.15	3.91
Completeness [%]	99.8	99.2
Redundancy	9.8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1 M Sodium citrate pH5.5, 20% PEG3K, VAPOR DIFFUSION, SITTING DROP, temperature 289K