3FGX

Structure of uncharacterised protein rbstp2171 from bacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97869
Spacegroup name	P 43
Unit cell lengths	89.099, 89.099, 31.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.090 - 2.900
R-factor	0.256
Rwork	0.255
R-free	0.27900
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.771
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.5.0051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.090	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.068	0.501
Number of reflections	5778
<I/σ(I)>	25.96	2.7
Completeness [%]	99.6	100
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1M HEPES sodium salt, 2% PEG 400, 2.0 M Ammonium sulfate , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K