3FGP
2.05 a Crystal Structure of CysM from Mycobacterium Tuberculosis - Open and Closed Conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.93400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.933, 89.142, 99.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| R-factor | 0.20041 |
| Rwork | 0.198 |
| R-free | 0.24671 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dki |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.150 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.086 | 0.637 |
| Number of reflections | 41528 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1M Tris-HCl pH 7.5, 0.1M K2HPO4, 4.3M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
